Intro to NGS processing

Who am I?

Who am I?

• Education
  • B.Sc. Bioarchaeology (University of York, UK)
  • M.Sc. Naturwissenschaftliches Archäologie (University of Tübingen, DE)
  • Ph.D. Archaeogenetics (MPI-SHH / MPI-EVA, DE)
• Experience
  • Number of genetics classes taken: 0
  • Number of bioinformatics classes taken: 0

@jfy133

Today we will

1. Introduce what NGS sequencing is
2. How do you generate Illumina NGS sequencing data
3. [Practical] How to evaluating NGS data

What is Sequencing?

Converting the chemical nucleotides of a DNA molecule

to

ACTG on your computer screen

What is NGS?

NGS: Next Generation Sequencing

• MASSIVELY multiplexed!
• Sequence millions and millions and millions and millions of DNA reads at once!

Not really ‘next’ anymore, consider it more ‘second’ generation (see: Nanopore)

Market leader:

How does it work?

cat 1.fastq 2.fastq